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Advantages of STED imaging using LBL-Dye Mito 715
STED (stimulated emission depletion) microscopy offers several advantages over confocal microscopy:
- Higher resolution: Achieves super-resolution imaging beyond the diffraction limit of light, allowing for sharper and more detailed images.
- Improved contrast: Enhances contrast by eliminating fluorescence from the outer regions of the excitation spot, resulting in clearer images of fine structures.
- Faster imaging: Allows for faster acquisition times compared to confocal microscopy, enabling the imaging of dynamic processes with higher temporal resolution.
- Multicolor imaging: Efficiently separates fluorescent signals from multiple dyes, facilitating multicolor imaging of complex biological systems.
- Reduced photobleaching and phototoxicity: Uses lower excitation power, minimizing photobleaching and phototoxic effects on samples.
STED microscopy provides unique advantages for high-resolution imaging with improved contrast and faster acquisition.
Input of STED nanoscopy on living animal cells labeled with 2 µg/µl of LBL-Dye Mito715.
Confocal (left) and STED (right) (20% power of 775-nm depletion laser) imaging.
Confocal
STED
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The efficacy of fluorescence-guided surgery in facilitating the real-time delineation of tumours depends on the optical contrast of tumour tissue over healthy tissue. Here we show that CJ215—a commercially available, renally cleared carbocyanine dye sensitive to apoptosis, and with an absorption and emission spectra suitable for near-infrared fluorescence imaging (wavelengths of 650–900 nm) and shortwave infrared (SWIR) fluorescence imaging (900–1,700 nm)—can facilitate fluorescence-guided tumour screening, tumour resection and the assessment of wound healing...
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The efficacy of fluorescence-guided surgery in facilitating the real-time delineation of tumours depends on the optical contrast of tumour tissue over healthy tissue. Here we show that CJ215—a commercially available, renally cleared carbocyanine dye sensitive to apoptosis, and with an absorption and emission spectra suitable for near-infrared fluorescence imaging (wavelengths of 650–900 nm) and shortwave infrared (SWIR) fluorescence imaging (900–1,700 nm)—can facilitate fluorescence-guided tumour screening, tumour resection and the assessment of wound healing...
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