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Cell-to-cell communication via tunneling nanotubes (TNTs) is a challenging topic with a growing interest. In this work, we proposed several innovative tools that use red/near-infrared dye labeling and employ lifetime-based imaging strategies to investigate the dynamics of TNTs in a living mesothelial H28 cell line that exhibits spontaneously TNT1 and TNT2 subtypes...

Studying mitochondrial dynamics within tunneling nanotubes (TNTs) through live cell imaging presents several challenges and requires sophisticated techniques to capture the intricacies of these dynamic structures...

Cellular tracers in fluorescence imaging are essential tools for studying various cellular processes, including cell migration, protein trafficking, organelle dynamics, and more...

A cell migration assay is a laboratory technique used to study how cells move in response to stimuli. It's crucial for understanding processes like tissue repair and cancer metastasis...

STED (stimulated emission depletion) microscopy offers several advantages over confocal microscopy: Higher resolution, improved contrast, faster imaging, multicolor imaging, reduced photobleaching and phototoxicity...

Labeling dyes can be used to visualize mitochondrial dynamics in living cells including mitochondrial fusion, fission, and movement. Combining it with time-lapse imaging can offer insights into mitochondrial trafficking, interactions with other cellular components, and their involvement in cellular processes...

Labeling dyes can be used to monitor spermatozoids in the cervix of animals. These dyes specifically stain the sperm cells, allowing easier visualization and tracking. This technique provides valuable insights into sperm transport and viability, aiding in the optimization of breeding strategies in animals...

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

Fluorescence microscopy is essential for a detailed understanding of cellular processes...

WMIC 2018 – CJ215, a new ultrasensitive near-infrared fluorescent probe for enhanced tumor detection in vivo: a comparative study with ICG in a preclinical model

Orthotopic patient-derived xenograft (PDX) pancreatic tumor models...