Phone
+33 (0)1 76 54 08 64

Email
contact@proimaging.fr

Address
66 avenue des Champs-Élysées
75008 Paris – France

Advantages of STED imaging using LBL-Dye M715

STED (stimulated emission depletion) microscopy offers several advantages over confocal microscopy:

  1. Higher resolution: STED microscopy achieves super-resolution imaging beyond the diffraction limit of light, allowing for sharper and more detailed images.
  2. Improved contrast: STED microscopy enhances contrast by eliminating fluorescence from the outer regions of the excitation spot, resulting in clearer images of fine structures.
  3. Faster imaging: STED microscopy allows for faster acquisition times compared to confocal microscopy, enabling the imaging of dynamic processes with higher temporal resolution.
  4. Multicolor imaging: STED microscopy efficiently separates fluorescent signals from multiple dyes, facilitating multicolor imaging of complex biological systems.
  5. Reduced photobleaching and phototoxicity: STED microscopy uses lower excitation power, minimizing photobleaching and phototoxic effects on samples.

STED microscopy provides unique advantages for high-resolution imaging with improved contrast and faster acquisition.

Input of STED nanoscopy on living animal cells labeled with 2 µg/µl of LBL-Dye M715.

Confocal (left) and STED (right) (20% power of 775-nm depletion laser) imaging.

Confocal

STED

Product citations :

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

Read more

Input of STED nanoscopy on living animal cells labeled with LBL-Dye M715..

Proimaging provided fluorescent labeling dyes LBL-Dye M715 and LBL-Dye M717 to the Primacen cell imaging research platform for…

Proimaging provided fluorescent labeling dyes to INRAE for a study on cervical sperm…

Proimaging