STED (stimulated emission depletion) microscopy offers several advantages over confocal microscopy:

1. Higher resolution: Achieves super-resolution imaging beyond the diffraction limit of light, allowing for sharper and more detailed images.
2. Improved contrast: Enhances contrast by eliminating fluorescence from the outer regions of the excitation spot, resulting in clearer images of fine structures.
3. Faster imaging: Allows for faster acquisition times compared to confocal microscopy, enabling the imaging of dynamic processes with higher temporal resolution.
4. Multicolor imaging: Efficiently separates fluorescent signals from multiple dyes, facilitating multicolor imaging of complex biological systems.
5. Reduced photobleaching and phototoxicity: Uses lower excitation power, minimizing photobleaching and phototoxic effects on samples.

STED microscopy provides unique advantages for high-resolution imaging with improved contrast and faster acquisition.

Input of STED nanoscopy on living animal cells labeled with 2 µg/µl of LBL-Dye M715.

Confocal (left) and STED (right) (20% power of 775-nm depletion laser) imaging.

Product citations :

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies