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Cell migration assay using LBL-Dye M580 and LBL-Dye M717

A cell migration assay is a laboratory technique used to study how cells move in response to stimuli. It's crucial for understanding processes like tissue repair and cancer metastasis.

Common assays include:

  • the scratch/wound healing assay, where a gap is created and closure is monitored;
  • the transwell assay, where cells migrate through a porous membrane toward a chemoattractant;
  • time-lapse microscopy for dynamic analysis.

These assays help researchers explore factors influencing cell movement and are essential for various biological studies.

LBL-Dye M580 and LBL-Dye M717 are two Cellular Tracers particularly well-adapted for cell migration assays.

After 2 days of acquisition, cells continue to divide and move. We visualize the cellular movements and the mixture of cells that are carried out.

No phototoxicity, no photobleaching, and no dye passive transfer are visible for all acquisition time.

Live-time imaging of human epithelial cell line with wide field microscope (Celdiscoverer 7, Zeiss) with 20X objective. Image acquisition every 10 min during few hours (3 days for entire time acquisition). Cells are cultured in “Culture-Insert 2 Well in µ-Dish 35 mm” (Ibidi) for 24 hrs. LBL-Dye M580 (4 µg/ml, Cyan) and LBL-Dye M717 (2 µg/ml, Majenta) are incorporated in each well during 1 hr; the cells outside of the insert are not labeled. After 3 rinses, insert is removed, and cell contact is followed for 3 days. Time image interval: 10 min. Transmitted light; Exc 560, Em 592/25 for LBL-Dye M580; Exc 650, Em 709/100 for LBL-Dye M717.

Product citations :

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

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A pan-cancer dye for solid-tumour screening, resection and wound monitoring via short-wave and near-infrared fluorescence imaging

The efficacy of fluorescence-guided surgery in facilitating the real-time delineation of tumours depends on the optical contrast of tumour tissue over healthy tissue. Here we show that CJ215—a commercially available, renally cleared carbocyanine dye sensitive to apoptosis, and with an absorption and emission spectra suitable for near-infrared fluorescence imaging (wavelengths of 650–900 nm) and shortwave infrared (SWIR) fluorescence imaging (900–1,700 nm)—can facilitate fluorescence-guided tumour screening, tumour resection and the assessment of wound healing...

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An optimized Nurr1 agonist provides disease-modifying effects in Parkinson’s disease models

The nuclear receptor, Nurr1, is critical for both the development and maintenance of midbrain dopamine neurons, representing a promising molecular target for Parkinson’s disease (PD)...

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Combining sophisticated fast FLIM, confocal microscopy, and STED nanoscopy for live-cell imaging of tunneling nanotubes

Cell-to-cell communication via tunneling nanotubes (TNTs) is a challenging topic with a growing interest. In this work, we proposed several innovative tools that use red/near-infrared dye labeling and employ lifetime-based imaging strategies to investigate the dynamics of TNTs in a living mesothelial H28 cell line that exhibits spontaneously TNT1 and TNT2 subtypes...

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