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LBL-Dye M715: High-performance tool for mitochondrial labeling in living cells

Labeling dyes can be used to visualize mitochondrial dynamics in living cells including mitochondrial fusion, fission, and movement. Combining it with time-lapse imaging can offer insights into mitochondrial trafficking, interactions with other cellular components, and their involvement in cellular processes. 

LBL-Dye M715 is a cell-permeant, photostable, and no cytotoxic mitochondrial tracker particularly well adapted for long time imaging on living cells.

Mitochondrial dynamic in H28 cell cytoplasms stained with LBL-Dye M715. Living H28 cells were stained with 2µg/µl of LBL-Dye M715 during 30 min in the culture medium. Time-lapse acquisition during 1 min every 3 sec by using STELLARIS8 confocal microscope (Leica Microsystems), X86 objective water immersion, WLL laser excitation 690nm ; Em 700-730 nm on HyD-X detector, Okolab chamber installed on the inverted microscope stand to keep the temperature at 37 ◦C during image acquisition.

Product citations:

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

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The efficacy of fluorescence-guided surgery in facilitating the real-time delineation of tumours depends on the optical contrast of tumour tissue over healthy tissue. Here we show that CJ215—a commercially available, renally cleared carbocyanine dye sensitive to apoptosis, and with an absorption and emission spectra suitable for near-infrared fluorescence imaging (wavelengths of 650–900 nm) and shortwave infrared (SWIR) fluorescence imaging (900–1,700 nm)—can facilitate fluorescence-guided tumour screening, tumour resection and the assessment of wound healing...

The nuclear receptor, Nurr1, is critical for both the development and maintenance of midbrain dopamine neurons, representing a promising molecular target for Parkinson’s disease (PD)...

Cell-to-cell communication via tunneling nanotubes (TNTs) is a challenging topic with a growing interest. In this work, we proposed several innovative tools that use red/near-infrared dye labeling and employ lifetime-based imaging strategies to investigate the dynamics of TNTs in a living mesothelial H28 cell line that exhibits spontaneously TNT1 and TNT2 subtypes...

Proimaging