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Mitochondrial dynamic visualized in the H28 cell cytoplasms labeled with LBL-Dye M715 and LBL-Dye M717

Proimaging provided fluorescent labeling dyes LBL-Dye M715 and LBL-Dye M717 to the Primacen cell imaging research platform for an article on new methods of live cell imaging with red/near-infrared dyes.

Living H28 cells were stained with 2µg/µl of LBL-Dye M715 during 30 min in the culture medium.
Time-lapse acquisition during 1 min every 3 sec by using STELLARIS8 confocal microscope (Leica Microsystems), X86 objective water immersion, WLL laser excitation 690nm ; Em 700-730 nm on HyD-X detector, Okolab chamber installed on the inverted microscope stand to keep the temperature at 37 ◦C during image acquisition.

Input of FLIM in STED nanoscopy of living H28 cells labeled with red/near-infrared dyes. Confocal (A1) and STED (20% 775-nm depletion laser, (A2)) imaging of LBL-Dye M717-labeled H28 cells (ex 690 nm, 3%, HyD-X). (A3A6) Impact of τ Strength factors (50–200) on STED imaging. Red box indicates best parameters configuration. Significant lateral resolution differences between imaging approaches (* p < 0.05 vs confocal approach) were determined using an ANOVA test followed by a Tukey–Kramer multiple comparison test.

Product citations:

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

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