Scratch assay using LBL-Dye M580 and LBL-Dye M717

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Scratch assay using LBL-Dye M580 and LBL-Dye M717

A scratch assay is a widely used laboratory technique to study the ability of cells to migrate and close a “scratch” or “wound” made in a monolayer of cultured cells. This assay is valuable for studying cell migration, a fundamental process in tissue repair, development, and disease progression.

LBL-Dye M580 and LBL-Dye M717 are two Cellular Tracers particularly well-adapted for cell migration assays.

After 2 days of acquisition, cells continue to divide and move. We visualize the cellular movements and the mixture of cells that are carried out.

No phototoxicity, no photobleaching, and no dye passive transfer are visible for all acquisition time.

**Live-time imaging of human epithelial cell line with wide field microscope** (Celdiscoverer 7, Zeiss) with 20X objective. Image acquisition every 10 min during few hours (3 days for entire time acquisition). Cells are cultured in “Culture-Insert 2 Well in µ-Dish 35 mm” (Ibidi) for 24 hrs. LBL-Dye M580 (4 µg/ml, Cyan) and LBL-Dye M717 (2 µg/ml, Majenta) are incorporated in each well during 1 hr; the cells outside of the insert are not labeled. After 3 rinses, insert is removed, and cell contact is followed for 3 days. Time image interval: 10 min. Transmitted light; Exc 560, Em 592/25 for LBL-Dye M580; Exc 650, Em 709/100 for LBL-Dye M717.

Product citations :

Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

Scratch assay using LBL-Dye M580 and LBL-Dye M717

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