+33 (0)1 76 54 08 64
66 avenue des Champs-Élysées
75008 Paris – France
Cellular tracers in fluorescence imaging are essential tools for studying various cellular processes, including cell migration, protein trafficking, organelle dynamics, and more…
LBL-Dye M580 and LBL-Dye M717 (compatible with super-resolution STED imaging) are two cell-permeant, photostable, and no cytotoxic cellular tracers particularly well-adapted for long-time imaging on living cells.
Imaging of H28-living cells stained with far-red LBL-Dye M580 (2µg/µl) in the culture medium. Images acquired by using STELLARIS8 confocal microscope (Leica Microsystems), X86 objective water immersion, WLL laser excitation 562 nm; Em 570-650 nm on HyD-S detector, Okolab chamber installed on the inverted microscope stand to keep the temperature at 37 ◦C during image acquisition.
Imaging of H28-living cells stained with far-red LBL-Dye M717 (5 µg/µl) in the culture medium. Images acquired by using SP5 confocal microscope (Leica Microsystems), X63 objective oil immersion, WLL laser excitation 690 nm; Em 700-730 nm. The plasma membrane and vesicles are visible on living cells and the movement of cells can be followed during few hours.
A scratch assay is a widely used laboratory technique to study the ability of cells to migrate and close a “scratch” or “wound” made in a monolayer of cultured cells. This assay is valuable for studying cell migration, a fundamental process in tissue repair, development, and disease progression…